Observations on Certain Enzyme Activities of Normal Human Red Cells Treated with Sulfhydryl Compounds
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چکیده
منابع مشابه
The Action of Two Sulfhydryl Compounds on Normal Human Red Cells. Relationship to Red Cells of Paroxysmal Nocturnal Hemoglobinuria.
characteristic lesion of the erythrocytes in the laboratory. Attempts along this line were made by Rodbard1 who, assuming that the erythrocytic defect was localized in the lipid fraction of the stroma, succeeded on a few occasions in obtaining lysis of normal red cells in acidified serum-which occurs also under the same conditions to PNH red cells2-by means of repeated washing with dilute alcoh...
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Evidence is presented that the activity of the hexose monophosphate (HMP) pathway of red cells, assayed by r4C02 production from glucose-lJ4C, is regulated primarily by glutathione. Methemoglobin had little, if any, effect on the activity of this pathway. Increasing the ratio of oxidized to reduced glutathione, either by peroxidizing GSH to GSSG or by partially blocking GSH with NEM, increased ...
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The morphology of red cells damaged by 2-aminoethylisothiouronium bromide (AET) has been compared to that of PNH cells by transmission and scanning electron microscopy. The main features of the effect of AET as demonstrated by the scanning electron microscope were spherical and deformed cells, with surface craters and relatively large pits. The transmission electron microscope revealed loss of ...
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One sulfhydryl group was modified per mol of native human gastric lipase after incubation at pH 8.0 with 5,5'-dithiobis(2-nitrobenzoic acid) for 18 h or with 4,4'-dithiopyridine for 100 min. With both reagents a direct correlation was found between the modification of one sulfhydryl group and the loss of human gastric lipase activity. Incubation of human gastric lipase with a new hydrophobic su...
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Fusion of neuraminidase or trypsin pretreated human erythrocytes and untreated cells was performed. The obtained doublets had a permanent dipole moment which disappeared as a function of time. Since the dipole moment disappearance is due to protein interdiffusion the lateral diffusion constant may be determined. This was performed by fixing fused cells in different time intervals following the ...
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ژورنال
عنوان ژورنال: Blood
سال: 1966
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v28.1.98.98